Current Issue : April - June Volume : 2011 Issue Number : 2 Articles : 13 Articles
Rabeprazole is an acid suppressant drug used in the treatment of peptic ulcer whereas domperidone is an anti emetic drug used in the treatment of emesis. This review explains the work done by different researchers so far for estimation of both these drugs alone or in combinations. Therefore, this review gives compiled data on both rabeprazole and domperidone together....
A selective and sensitive spectrophotometric method for determination of Granisetron has been developed and validated by three simple, precise and economical UV spectrophotometric methods have been developed for the estimation of Granisetron in bulk and tablet dosage form. A review of the literature revealed no methods for determination of granisetron in pharmaceutical dosage forms by UV spectrophotometric methods and previously reported methods were mainly focused on the analysis of this drug in biological fluids which includes HPLC methods with fluorescence detection (1-4) and LC-MS/MS methods(5-6) for determination of granisetron in human biological fluids. The BP(7) describes an HPLC method for the determination of Granisetron in bulk i.e. raw material. Since the UV spectrophotometric methods are simple and fast for routine analysis. Granisetron has the absorbance maxima at 302 nm (Method A), and in the first order derivative spectra showed sharp peak at 291 nm (Method B). Method C applied was in the wavelength range of 298-312 nm. Linearity for detector response was observed in the concentration range of 1-12μg/mL for Method A, Method B and Method C. This method was validated according to validation procedures, parameters and acceptance criteria based on USP XXIII guidelines and recommendations of Shah et al.(8-9). The proposed methods were successfully applied for the simultaneous determination of Granisetron in commercial tablet preparation....
A binary mixture of Levofloxacin and Ornidazole was determined by RP-HPLC method. The method involved determination of Levofloxacin and Ornidazole by reversed-phase liquid chromatography using water: acetonitrile: triethylamine (75:25:0.1, v/v) and final pH adjusted to 3.15 ± 0.02 with 5% v/v ortho-phosphoric acid and was pumped at a flow rate of 1.5 ml/min. Quantification was achieved with ultraviolet detection at 310 nm over concentration ranges of 0.5-8.0 µg/ml and 1-16 µg/ml; mean accuracies were 98.74 - 100.5 and 98.89 - 102.3%, respectively. The method was successively applied to pharmaceutical formulation because no chromatographic interferences from the tablet excipients were found. The method retained its accuracy and precision when the standard addition technique was applied....
The present work describes a validated reverse phase high performance liquid chromatographic method for simultaneous estimation of Diclofenac sodium and Famotidine in pharmaceutical dosage form. Chromatography was performed on a Varian C18 column (250 mm x 4.6 mm i.d., 5 µm particle size), column with mobile phase containing acetonitrile, methanol and water in the ratio of 25:30:45 v/v.The flow rate was 1.0 ml/min and the eluent was monitored at 283 nm. The selected chromatographic conditions were found to effectively separate Diclofenac sodium (RT- 2.432 min) and Famotidine (RT- 5.082 min). Linearity for Diclofenac sodium and Famotidine were found in the range of 5-35μg/ml and 2-14μg/ml. The values obtained of LODs were 0.036µg/ml and 0.028µg/ml; LOQs were 0.11µg/ml and 0.088µg/ml for Diclofenac sodium and Famotidine, respectively. The proposed method was found to be fast, accurate, precise, reproducible and rugged and can be used for simultaneous analysis of Diclofenac sodium and Famotidine in combined pharmaceutical formulations....
The present work describes a validated RP-HPLC method for simultaneous estimation of Ranitidine HCL (RANI) and Drotaverine (DROTA) in combined dosage form. The method was performed on a Gradient HPLC instrument using Water: Methanol: Acetonitrile (10:80:10 v/v/v), pH adjusted at 8.5 with Diethyl amine as a mobile phase. Areas were recorded at 324 nm for both the drugs and retention time was found at 2.76 min and 4.58 min for Ranitidine and Drotaverine respectively at 1.0 ml/min flow rate. The selected chromatographic conditions were found to quantitatively determine Ranitidine and Drotaverine in a combined dosage form without any physical separation. Linearity was found over the range of 5-50 μg/ml for Ranitidine and 2-12 μg/ml for Drotaverine. The values of Limit of Detection were found to be 0.077 μg/ml for Ranitidine and 0.215 μg/ml Drotaverine. The values of Limit of Quantition were found to be 0.232 μg/ml for Ranitidine and 0.652 μg/ml for Diclofenac. The proposed method was found to be fast, accurate, precise, reproducible and rugged and can be used for simultaneous analysis of these drugs in tablet....
A simple and precise High-performance thin-layer chromatographic (HPTLC) method for determination of amlodipine besylate (AMB) and perindopril erbumine (PER) in their bulk and tablet dosage forms has been developed and validated. This employs a precoated silica gel 60F254 on aluminium sheets and mobile phase was Chloroform: Ethanol: Toluene: Glacial acetic acid in the ratio of 5:3:3.5:0.5v/v. having chamber saturation for 20 minutes at room temperature. The TLC plate was run up to 8 cm. Detection was performed at 230 nm. The RF values were 0.15 for amlodipine and 0.85 for perindopril. The developed method validated for its accuracy and precision. The LOD and LOQ were found to be 0.1 μg/ml and 0.3 μg/ml, 0.1 μg/ml and 0.3 μg/ml respectively for amlodipine and perindopril. The recovery was carried out by standard addition method. The Average recovery was found to be 99.22% and 100.73% for amlodipine and perindopril respectively....
Andrographis paniculata Nees popularly known as “Kalmegh” used in indigenous system of medicine as a hepatoprotective drug.In the present investigation, pharmacognostical, physicochemical characteristics, thin layer chromatography, and HPLC studies of Andrographis paniculata Nees were studied. In order to ensure the use of only genuine and uniform material of such herbal remedies, work on standardization assumes vital significance.Preliminary phytochemical analysis showed that alkaloids, flavonoids, glaycosides,, saponins, steroids, tannins, were present. The results of the pharmacognostical studies, features of powdered drug include response to U.V. Light exposure, some physicochemical constants, results of TLC, and chromatogram of HPLC, would serve as standard reference for identifications of Andrographis paniculata (Nees)....
A simple, sensitive, precise and specific RP-HPLC method was developed and validated for the estimation of Glimepiride in tablet dosage form. The method was performed on a RP-HPLC (Shimadzu LC-2010C HT) model, Symmetry C18- column, 250 mm × 4.6 mm, 5 µm particle size. The mobile phase was mixture of sodium dihydrogen phosphate adjusted to the pH 2.5 with orthophosphoric acid and acetonitrile (40:60) at a flow rate of 1 ml/min at ambient temperature. UV detection was performed at 228 nm. The linear response was observed in the range of 50 to 150µg/ml with a correlation coefficient of 0.999996. System precision and method precision were found to be within limits and also ruggedness. The proposed method has adequate specificity for the estimation of Glimepiride in tablet dosage form. The percentage recovery was found to be between the ranges of 99.33% to 100.59%. The method was validated statistically and was applied successfully for the estimation of Glimepiride in tablet dosage form....
The influence of three different solvents on estimation of lapatinib ditosylate from bulk and pharmaceutical dosage forms was studied. The solutions of lapatinib ditosylate were prepared employing pure organic solvents namely methanol, ethanol and also in pH2.0 phosphate buffer. The solutions were scanned with in the ranges of 200 nm to 400nm. Lapatinib exhibited λmax at 263 nm in case of methanol and ethanol; however the λmax was slightly shifted to 268.5nm in pH2.0 phosphate buffer. The method obeyed Beer-Lamberts law with in the range of 5-35μg/ml concentration. The analytical parameters such as linearity, A (1%, 1 cm), LOD, LOQ, inter day precision, intraday precision, molar absorption coefficient, and sandells sensitivity were calculated. The results of analysis were validated by recovery studies. The recovery was more than 98%. The method was found to be simple, accurate, precise, economical and robust. The validation parameters were treated statistically with ANOVA and there were significant differences noticed. This study clearly indicated that the solvent is having any influence in determination of lapatinib from bulk drug as well as from the formulations. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of lapatinib ditosylate from pharmaceutical preparation....
A simple, sensitive, precise & stability indicating RP-HPLC method was developed & validated for the determination of Tramadol hydrochloride in tablet dosage form. The chromatographic conditions comprised of column XTerra�® C 18 column (250 mm, id 4.6 mm, 5 �µm),Water Ireland using mobile phase phosphate buffer : acetonitrile (75:25 v/v) at a flow rate of 1.5 ml/min at an ambient temperature. The retention time of tramadol was 6.49 min. Tramadol hydrochloride was subjected to acid & alkali hydrolysis, oxidation, photochemical determination & thermal degradation. The degraded product was well separated from the drug. The linear regration analysis data for the calibration plots showed regration value 0.9999 in the concentration range 200.60-601.80.The value of slope & intercept were 20.87 & -6.87 respectively. The method was validated for precision, recovery, ruggedness & robustness. The drug undergoes degradation under acidic, basic, photochemical & thermal....
Two simple, accurate, and reproducible UV spectrophotometric methods for simultaneous estimation of Amoxicillin Trihydrate (AMOX) and Ambroxol Hydrochloride (AMBRO) have been developed in the present work. The first developed method was Q-ratio method, wavelength selected are 228nm (λmax of amoxicillin) and 250nm (iso-absorptive point for both drugs). The second developed method was AUC with wavelength ranges of 223-233 nm and 240-250 nm selected to determine AMOX and AMBRO. Linearity for both the methods was observed in concentration range of 2-12µg/ml for AMBRO and 20-120 µg/ml for AMOX. The correlation coefficients for both the methods obtained were near to 1. The accuracy of these methods were evaluated by recovery studies and good recovery results were obtained from 98 % to 102% for both the methods and the relative standard deviation of below 2% were achieved as per ICH guidelines....
Atazanavir sulphate (ATV) is a new HIV-1 protease inhibitor anti retroviral drug. The present study describes a simple, accurate, reproducible and precise UV Spectrophotometric method for the estimation of ATV in 0.025 N HCl. ATV is insoluble in 0.025 N HCl, so methanol was used as a cosolvent. The absorbance maximum (λmax) was found to be 300 nm with linearity in the range of 5-60µg/ml and regression equation as Y = 0.013-0.001. Regression coefficient was calculated as 0.999. The method was validated for different parameters such as sandell’s sensitivity, molar absorptivity, accuracy, precision, ruggedness, robustness, detection limit, quantification limit, etc (as per the ICH guidelines). Molar absorption coefficient and sandell’s sensitivity comes out to be 1.06 X 104 l.mol-1cm-1 and 0.1538µg/ml/0.001 A.U. respectively. LOD and LOQ were found to be 0.2071µg/ml and 0.627µg/ml respectively. The relative standard deviation (RSD) in case of accuracy, precision, ruggedness and robustness was less than 2.0% proving that method was highly accurate, precise and robust. This method can be used for the determination of ATV in pharmaceutical formulations without interference of the excipients....
The present research work discusses the development of a UV spectrophotometric method for Bromelain. Simple, sensitive, reproducible, accurate and cost efficient spectrophotometric method has been developed for the estimation of Bromelain in bulk. The optimum conditions for the analysis of the drug were established. The study was conducted in phosphate buffer pH 6.8. The maximum wavelength (λmax) was found to be 280nm. The method validation was accomplished through evaluation of analytical parameters of linearity, range, accuracy and precision, limit of detection and limit of quantification and robustness as per ICH guidelines. The developed method was linear (R2= 0.997) in the concentration range of 100-1000 μg/ml, precise (%RSD inter day= 0.68, 0.35, 0.83 for three consecutive days, %RSD intraday= 0.79, 0.23, 1.62), accurate (% recovery= 98.75-99.27%) and sensitive (LOD and LOQ of 3.34 and 10.12 µg/ml respectively). The proposed method is observed to be suitable for the analysis of Bromelain in bulk for quality control purposes....
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